Thermostability of proteins refers to the ability of proteins to maintain their three-dimensional structures at high temperatures. PON-Tm is a predictor of the effect of missense mutations on the thermostability of proteins. The method is entirely sequence-based, thus allowing the use of the protein sequence as input rather than the protein structure. The method does not restrict protein types, thus enabling the input of all proteins for which a primary structure can be determined, including antibody proteins and membrane proteins.
There are two input formats. Sequences can be given either in FASTA format or indicated by their IDs.
Three version of MutDB, and the train/test datasets used in PON-Tm are freely available.
Results are displayed on the Results page based on your IP, and we recommend using email to receive results.
PON-Tm manuscript has been submitted. In the meantime if using the service cite the URL.
The original PON-Tm was published in Kuang, J., Zhao, Z., Yang, Y., & Yan, W. (2024). PON-Tm: A Sequence-Based Method for Prediction of Missense Mutation Effects on Protein Thermal Stability Changes. International Journal of Molecular Sciences, 25(15), 8379. https://doi.org/10.3390/ijms25158379. IJMS